evaluation of radiolabeled streptokinase for thrombosis imaging
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abstract
introduction: cardiovascular disease is the major cause of morbidity and mortality in developing and developed countries. rapid diagnosis of the thrombosis can be an essential step in management of the stroke. methods: in this work a recently developed radiolabeled streptokinase (stp) tracer was evaluated in an animal thrombotic model using spect imaging and biodistribution studies. locally labeled [67ga]-streptokinase was checked by itlc, hplc and sds-page experiments to check the tracer integrity and purity. the biodistribution studies were performed in thrombotic femoral vein of rats using tissue counting and preliminary spect studies, respectively (up to 2 h). results: [67ga]-streptokinase prepared with suitable radiochemical purity (hplc >95%, itlc >99%) was administered to fecl3 induced thrombotic rats and the percentage of injected dose per gram of tissue (id/g%) as well as the spect images demonstrated the good specific binding of the tracer in thrombotic clots located in heart and aorta 2 hours post injection.conclusion: [67ga]-dtpa-stp can be a suitable probe for imaging of thrombosis in cardiovascular diseases. ga-68 labeled stp has the potential to be an alternative superior labeled compound due to positron emission properties for pet studies as well as appropriate physical half life.
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Evaluation of radiolabeled streptokinase for thrombosis imaging
Introduction: Cardiovascular disease is the major cause of morbidity and mortality in developing and developed countries. Rapid diagnosis of the thrombosis can be an essential step in management of the stroke. Methods: In this work a recently developed radiolabeled streptokinase (STP) tracer was evaluated in an animal thrombotic model using SPECT imaging and biodis...
full textEvaluation of Radiolabeled Streptokinase for Thrombosis Imaging
Introduction: Cardiovascular disease is the major cause of morbidity and mortality in developing and developed countries. Rapid diagnosis of the thrombosis can be an essential step in management of the stroke. Methods: In this work a recently developed radiolabeled streptokinase (STP) tracer was evaluated in an animal thrombotic model using SPECT imaging and biodistribution studies. Locally lab...
full textPreparation and quality control of radiolabeled streptokinase for Thrombosis imaging
Background: In order to diagnose the site of thrombi, radiolabeled streptokinase can be prepared. The radiolabeled compound can be used in imaging of thrombi in many cardiovascular diseases. Materials and Methods: Streptokinase was successively labeled with [67Ga]-gallium chloride using cyclic DTPA-dianhydride. The conjugation with DTPA was optimized for concentration, time and temperature foll...
full textpreparation and quality control of radiolabeled streptokinase for thrombosis imaging
background: in order to diagnose the site of thrombi, radiolabeled streptokinase can be prepared. the radiolabeled compound can be used in imaging of thrombi in many cardiovascular diseases. materials and methods: streptokinase was successively labeled with [67ga]-gallium chloride using cyclic dtpa-dianhydride. the conjugation with dtpa was optimized for concentration, time and temperature foll...
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Journal title:
iranian journal of nuclear medicinePublisher: tehran university of medical sciences
ISSN 1681-2824
volume 16
issue 2 2008
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